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Life Extension Magazine




May 5, 2000

 

References


 

161. Immunostimulation by vitamin C.
Int J Vitam Nutr Res Suppl 1982;23:49-52
Banic S

A review of the literature shows that vitamin C undoubtedly has a positive effect on some mechanisms of the natural resistance against infections as well as on the immune response. It is generally accepted that vitamin C increases the chemotaxis of granulocytes and of macrophages. There is also ample evidence that vitamin C increases the phagocytic activity of granulocytes as well as of macrophages. As to the effect of vitamin C on the immune response, the available data allow the conclusion that vitamin C is necessary for the induction of delayed hypersensitivity, i.e. cellular immunity. Its effect on the antibody production appears, however, to be minimal or none. In our previous in vivo studies we obtained the results which were consistent with the conclusion that high doses of vitamin C increase the efficiency of immunization with a human cell culture rabies vaccine in guinea-pigs. The purpose of this study was to establish whether relatively low doses (10 mg/kg body weight) of vitamin C have a similar effect as megadoses (100 mg/kg body weight). We observed that the efficiency of immunization with the human diploid cell culture rabies vaccine (Merieux/Lyon) was better in the group of guinea-pigs treated with the vaccine plus vitamin C (10 mg/kg body weight) than in the group treated with the vaccine alone. The difference in the number of survivors in both groups of animals was statistically not significant, but considering the factor of repetition (a similar result in repeated experiments) we nevertheless conclude that vitamin C acts in the doses used as an immunopotentiator.

162. J Bioenerg Biomembr 1994 Aug;26(4):349-58
The role of ascorbate in antioxidant protection of biomembranes: interaction
with vitamin E and coenzyme Q.
Beyer RE
Department of Biology, University of Michigan, Ann Arbor 48109.

One of the vital roles of ascorbic acid (vitamin C) is to act as an antioxidant
to protect cellular components from free radical damage. Ascorbic acid has been
shown to scavenge free radicals directly in the aqueous phases of cells and the
circulatory system. Ascorbic acid has also been proven to protect membrane and
other hydrophobic compartments from such damage by regenerating the antioxidant form of vitamin E. In addition, reduced coenzyme Q, also a resident of hydrophobic compartments, interacts with vitamin E to regenerate its antioxidant form. The mechanism of vitamin C antioxidant function, the myriad of pathologies resulting from its clinical deficiency, and the many health benefits it
provides, are reviewed.

163. Int J Epidemiol 2000 Apr;29(2):260-5
Saturated fat, vitamin C and smoking predict long-term population all-cause
mortality rates in the Seven Countries Study.
Kromhout D, Bloemberg B, Feskens E, Menotti A, Nissinen A
Division of Public Health Research, National Institute of Public Health and the
Environment, Bilthoven, The Netherlands. daan.kromhout@RIVM.nl

BACKGROUND: The Seven Countries Study has shown that population mortality rates for various chronic diseases are related to diet and smoking. This paper
addresses the associations between diet, smoking and 25-year all-cause
mortality. METHODS: Baseline surveys were carried out between 1958 and 1964 on 12,763 middle-aged men constituting 16 cohorts in seven countries. In 1987/88 equivalent food composites representing the average food intake of each cohort at baseline were collected and chemically analysed in one central laboratory. During 25 years of follow-up 5973 men died and age-adjusted population mortality rates were calculated for each cohort. RESULTS: Multivariate linear regression analyses showed that the population intake of saturated fat and the prevalence of smoking were positively associated with population all-cause mortality rates. Population vitamin C intake was inversely associated with all-cause mortality. It was calculated that a reduction in saturated fat intake of 5% of energy, a 20 mg/d increase in vitamin C and a 10% decrease in the prevalence of smokers may decrease the 25-year all-cause population mortality rate by 12.4% (95% CI: 5.6, 19.4%) at an average population all-cause mortality rate of 45%. CONCLUSION: At the population level saturated fat, vitamin C and cigarette smoking are important determinants of all-cause mortality.

164. Nutr Rev 2000 Mar;58(3 Pt 1):73-5
Does vitamin C intake protect against lead toxicity?
Houston DK, Johnson MA
Department of Foods and Nutrition, Faculty of Gerontology, University of
Georgia, Athens 30602, USA.

Although several animal studies suggest a protective relationship between blood
lead concentrations and ascorbic acid, there are inconclusive results regarding
the beneficial effect of ascorbic acid on lead concentrations in human studies.
Data from the Third National Health and Nutrition Examination Survey examined
the association between ascorbic acid and blood lead concentrations in 19,578
participants ages 6-90 years without a history of lead poisoning. Elevated blood
lead concentrations were found in 0.4% of adults and 0.5% of youths. Serum
ascorbic acid concentrations were inversely associated with the prevalence of
elevated blood lead concentrations. However, there was no significant
relationship between dietary ascorbic acid intake and blood lead concentrations.
This study suggests that there may be a protective relationship between ascorbic
acid and lead. Questions remain regarding the unique roles of dietary vitamin C
versus supplemental vitamin C in explaining this relationship.

165. Cochrane Database Syst Rev 2000;2:CD000980
Vitamin C for preventing and treating the common cold.
Douglas RM, Chalker EB, Treacy B
National Centre for Epidemiology and Population Health, The Australian National
University, Canberra, ACT, AUSTRALIA, 0200. Bob.Douglas@anu.edu.au
[Record supplied by publisher]

BACKGROUND: The role of oral ascorbic acid (vitamin C) in the prevention and
treatment of colds remains controversial despite many controlled trials. There
have also been a number of efforts to synthesize and/or overview the results of
these trials, and controversy over what these overviews tell us. OBJECTIVES: The objective of this review was to answer the following two questions: (1) Does
regular high dosage supplementation with vitamin C reduce the incidence of
colds? (2) Does taking vitamin C in high doses at the onset of a cold have a
therapeutic effect? SEARCH STRATEGY: This review currently deals only with
published trials from two previously published reviews by Kleijnen 1989 and
Hemila 1992. SELECTION CRITERIA: Randomised and non-randomised trials of vitamin C taken to prevent or treat the common cold. DATA COLLECTION AND ANALYSIS: Two reviewers independently extracted data and assessed trial quality. MAIN RESULTS: Thirty trials were included. The quality of the included trials was variable. Vitamin C in doses as high as one gram daily for several winter months, had no consistent beneficial effect on incidence of the common cold. For both preventive and therapeutic trials, there was a consistently beneficial but generally modest therapeutic effect on duration of cold symptoms. This effect was variable, ranging from -0.07% to a 39% reduction in symptom days. The weighted difference across all of the studies revealed a reduction of a little less than half a symptom day per cold episode, representing an 8% to 9%
reduction in symptom days. There was no clear indication of the relative
benefits of different regimes or vitamin C doses. However in trials that tested
vitamin C after cold symptoms occurred, there was some evidence that a large
dose produced greater benefits than lower doses. REVIEWER'S CONCLUSIONS: Long term daily supplementation with vitamin C in large doses daily does not appear to prevent colds. There appears to be a modest benefit in reducing duration of cold symptoms from ingestion of relatively high doses of vitamin C. The relation of dose to therapeutic benefit needs further exploration.

166. Evidence for a recommended dietary allowance for vitamin C from pharmacokinetics: a comment and analysis.
Proc Natl Acad Sci U S A 1996 Dec 10;93(25):14344-
Young VR Laboratory of Human Nutrition, Clinical Research Center Massachusetts Institute of Technology, Cambridge 02139, USA.
The current recommended dietary allowance (RDA) for vitamin C, as proposed by the Food and Nutrition Board/National Research Council in 1980 and reconfirmed in 1989, is 60 mg daily for nonsmoking adult males. Levine et al. [Levine, M., Conry-Cantilena, C., Wang, Y., Welch, R. W., Washko, P. W., et al. (1996) Proc. Natl. Acad. Sci. USA 93, 3704-3709], based on a study of vitamin C pharmacokinetics in seven healthy men, have now proposed that the RDA should be increased to 200 mg daily. I have examined, in brief, the experimental and conceptual bases for this new recommendation and its implications for public health and nutrition policy and programs. Using, for illustrative purposes only, data extracted from each of two recent dietary surveys of noninstitutionalized adult males living in households in the Netherlands and the United States, it is predicted that the prevalence of intakes inadequate to meet the individual's own requirement would be about 96% or 84%, respectively, if the criteria of adequacy used for derivation of the 200 mg RDA are accepted. Depending upon the particular average requirement value for ascorbic acid that might be derived from their data, the proposal by Levine et al. would mean a desirable increase in mean intakes in these two populations by as much about 2-to 3-fold. Hence, before an action of this kind is to be recommended, an answer must be sought to the question whether

167. Selenium in ischaemic heart disease. Salonen JT Int J Epidemiol, 1987 Jun, 16:2, 323?8 Ecological between?area comparisons, hospital?based case?control studies and cross?sectional angiography studies have provided data on the role of selenium (Se) deficiency in the aetiology of ischaemic heart disease (IHD), but this evidence is, at best, suggestive because of the potential selection biases and the lack of certainty of the temporal direction of the observed associations. Out of the four cohort?based population studies published so far, one observed a moderate association between a reduced serum Se and an increased risk of IHD and all cardiovascular (CVD) deaths, one was equivocal and two did not find any association between serum Se and IHD. There are, however, several potential sources of biases acting towards the null hypothesis in these studies. In the pooled data of the two separate cohorts from eastern Finland including 377 people who experienced a CVD death or a non?fatal myocardial infarction and equally many event?free risk?factor matched controls, people with serum Se of less than 45 micrograms/l had a 1.7?fold (95% confidence interval 1.2?2.7) risk of an CVD event compared to those with higher serum Se. This association could, however, be explained by the covariation of Se with other nutrients, as for example n?3 polyunsaturated fatty acids. As the evidence concerning the role of serum Se in IHD is inconclusive, we need new epidemiological studies to test the association and experiments exploring the possible mechanisms.

168. Primary and secondary prevention of atherosclerosis: is there a role for antioxidants?
Paolisso G; Esposito R; DAlessio MA; Barbieri M
Institute of Cardiology, II University of Naples, Italy. gpaoliss@tin.it
Diabetes Metab, 1999 Sep, 25:4, 298?306

Elevated plasma free radical concentration (expression of enhanced oxidative stress) is related to different pathophysiological conditions such as ageing, cancer and diabetes. Nevertheless, even in healthy subjects a rise in plasma free radicals is due to hyperglycaemia, elevated free fatty acids and hyperinsulinaemia. Once elevated oxidative stress occurs, accelerated atherosclerosis may be present. Thus, antioxidants might potentially be useful in preventing or delaying the development of atherosclerosis. Several epidemiological studies have provided conflicting results, whereas interventional studies have demonstrated that antioxidant administration at pharmacological doses is useful for secondary prevention of atherosclerosis. The role of antioxidants in diabetic patients is still debatable, and it is too early to suggest this means for the prevention of atherosclerosis. Concerning trace elements, several studies have indicated that iron, copper, zinc and selenium may play a role in the pathogenesis of atherosclerosis. Nevertheless, only future longitudinal studies can provide a final response. In conclusion, the whole body of studies to date clearly demonstrates that antioxidants may be useful for secondary prevention of coronary heart disease.

169. "The SU.VI.MAX Study": a primary prevention trial using nutritional doses of antioxidant vitamins and minerals in cardiovascular diseases and cancers. SUpplementation on VItamines et Minéraux AntioXydants.
Hercberg S; Preziosi P; Galan P; Faure H; Arnaud J; Duport N; Malvy D; Roussel AM; Briançon S; Favier A
Institut Scientifique et Technique de la Nutrition et de l'Alimentation/CNAM, Paris, France.
Food Chem Toxicol, 1999 Sep, 37:9?10, 925?30

Study is a randomized double?blind, placebo?controlled, primary?prevention trial which started in 1994 in France. This epidemiologic study is designed to test the efficacy of a daily supplementation with antioxidant vitamins (vitamin C, 120 mg, vitamin E, 30 mg, and beta?carotene, 6 mg) and minerals (selenium, 100 microg, and zinc, 20 mg) at nutritional doses, in reducing the main causes of premature death (cancers and cardiovascular diseases); 12,735 eligible subjects (women aged 35 to 60 years, and men aged 45 to 60 years) were included in 1994 and will be followed up for 8 years. Participants undergo a yearly visit consisting, every other year, of either biological sampling or clinical examination. They also regularly provide information on health events and dietary intake by filling out computerized questionnaires using the Minitel Telematic Network. After 2 years of supplementation, biochemical indicators of vitamin and trace element status reach reasonable level without reaching concentrations as high as those observed in intervention studies, which tested relatively high doses of antioxidants, and ended up with higher risk of pathology.

170. Primary and secondary prevention of atherosclerosis: is there a role for antioxidants?
Paolisso G, Esposito R, D'Alessio MA, Barbieri M
Institute of Cardiology, II University of Naples, Italy. gpaoliss@tin.it
Diabetes Metab 1999 Sep;25(4):298?306

Elevated plasma free radical concentration (expression of enhanced oxidative stress) is related to different pathophysiological conditions such as ageing, cancer and diabetes. Nevertheless, even in healthy subjects a rise in plasma free radicals is due to hyperglycaemia, elevated free fatty acids and hyperinsulinaemia. Once elevated oxidative stress occurs, accelerated atherosclerosis may be present. Thus, antioxidants might potentially be useful in preventing or delaying the development of atherosclerosis. Several epidemiological studies have provided conflicting results, whereas interventional studies have demonstrated that antioxidant administration at pharmacological doses is useful for secondary prevention of atherosclerosis. The role of antioxidants in diabetic patients is still debatable, and it is too early to suggest this means for the prevention of atherosclerosis. Concerning trace elements, several studies have indicated that iron, copper, zinc and selenium may play a role in the pathogenesis of atherosclerosis. Nevertheless, only future longitudinal studies can provide a final response. In conclusion, the whole body of studies to date clearly demonstrates that antioxidants may be useful for secondary prevention of coronary heart disease.



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