Life Extension Magazine June 2005
By Dale Kiefer
Conjugated linoleic acid (CLA) is gaining the attention of a public grown ravenous for new weapons to wield in the never-ending “battle of the bulge.” You may have heard some of the hype, but what is the real story behind this relative newcomer to the weight-loss market? A growing body of research reveals that CLA is a potent nutrient responsible for a variety of important health benefits, from fighting cancer and diabetes to boosting fat loss and building lean muscle.
First hailed for its striking anti-cancer effects,1 CLA is now the subject of intensive research by scores of scientists around the globe, all of them intrigued by this nutrient’s multiple human health benefits. Accelerated fat loss is just one of several boons that have been ascribed to this fatty acid supplement. CLA’s other reported benefits include combating diabetes, building lean muscle mass, and thwarting cancer at each major stage of its development.1-7
While it may be too soon to render the final verdict on all of CLA’s purported benefits, the scientific evidence clearly supports some of its biological effects. In this article, we will examine the current state of CLA research, covering this important nutrient’s demonstrated effects and examining others that remain to be fully elucidated.
What Is CLA?
CLA comprises several chemically similar compounds, or isomers, derived from the fatty acid known as linoleic acid. Present in dairy products and the meat of ruminant animals—livestock such as cattle and sheep that chew their cud—CLA is believed to play an important role in modulating immunity and regulating lipid metabolism.2,5,8
The “conjugated” portion of this nutrient’s name refers to two double chemical bonds within its structure. The location of the bonds dictates a given isomer’s specific designation. For example, the cis-9, trans-11 CLA isomer (also known as rumenic acid, or c9t11) is the most common isomer found in ruminant-derived foods, and is believed to be one of the most biologically active isomers. Trans-10, cis-12 CLA (t10c12) is present in far smaller concentrations, but may play an especially important role in modulating health.4 These two isomers are considered the most biologically important forms of CLA for human health. Most commercially available CLA supplements include a roughly equal mixture of both.
CLA and Weight Loss
Early CLA experiments on laboratory rodents generated considerable surprise and excitement. Scientists discovered that CLA, when added to the diet, promotes dramatic and relatively rapid weight loss in a variety of lab animals. Perhaps more important, the animals lost body fat while increasing their lean muscle mass.
When researchers fed rabbits a diet that normally promotes atherosclerosis, the animals were considerably less likely to develop this artery-clogging disease when CLA was included in their diet. Their total cholesterol and LDL (low-density lipoprotein) levels dropped, as did their triglyceride values.9 Furthermore, using a strain of rats bred for a tendency to develop obesity-related type II diabetes, scientists were able to significantly delay the onset of diabetes by adding CLA to the animals’ diet.10
Needless to say, scientists were excited about CLA’s potential to fight fat in humans. Obesity is epidemic in much of the developed world, and the federal Centers for Disease Control and Prevention reports that approximately two thirds of American adults are now overweight or obese. Even more alarming is that 13% of American children and adolescents are overweight.11,12 All that excess tonnage takes a heavy toll, not only on individuals’ health but also on our collective bottom line. In 2002, the estimated total economic cost of obesity was about $93 billion.13
It is understandable that hopes for CLA ran high in those early days of model-thin mice and shrinking rats. However, CLA may not be quite the magic bullet scientists had hoped it would be. Although CLA triggers dramatic fat loss in rodents, the effect is less pronounced in humans. Some human clinical trials have reported promising results,14 while others have found little or no fat-loss effect.17 A few studies have reported adverse effects of CLA on lipid profiles, markers of oxidative stress, and glucose handling,14 while other long-term studies have not observed these negative effects.21
Thus, our understanding of CLA is in a state of flux. Much work remains to be done as we assess CLA’s potential benefits or risks. With that caveat, let us shed some light on what is known about this important nutrient.
CLA and Diabetes
CLA appears to prevent the development of type II diabetes (often associated with obesity) in rats bred for their propensity to grow abnormally overweight. Some researchers have proposed that the t10c12 isomer is primarily responsible for this anti-diabetic and anti-obesity effect.14
Human studies confirm the efficacy of the t10c12 isomer in promoting weight loss. For example, in 2003, Swedish researchers published the results of a series of controlled experiments in which subjects received either a CLA mixture or purified t10c12 isomer. The results indicated that CLA does reduce body fat, though no overall weight loss was detected. Unexpectedly, the pure t10c12 isomer appeared to adversely affect glucose and lipid levels, and to impair insulin sensitivity.14
Other research, however, suggests that CLA may improve the insulin sensitivity of fat cells, and may work in combination with nutrients such as biotin and coenzyme Q10 to prevent diabetes.15
A recent study showed that eight weeks of CLA supplementation increased insulin sensitivity in young, sedentary humans. Ten participants took 4 grams of CLA daily. Six of the subjects experienced large increases in insulin sensitivity, while two showed no change and two demonstrated a decrease in insulin sensitivity. These results suggest that response to CLA varies among individuals, with many responding favorably.16
In 2000, researchers in California conducted an experiment on 17 women who were confined to a metabolic laboratory for three months. Their activity levels and food intakes were strictly controlled. After a month of baseline monitoring, the subjects were given a daily supplement of 3 grams of either a CLA mixture or an inactive placebo. After two months of supplementation, the scientists concluded that CLA had no effect on body composition.17 The study’s design did not, however, account for the possibility that CLA may reduce body fat or weight by suppressing appetite, since all of the subjects in this trial were required to adhere to a uniform diet. It is also possible that the subjects simply did not consume enough CLA or did not consume it long enough to generate significant changes in body composition.
Reducing Body Fat
Other researchers have reported that CLA does indeed reduce body fat, if not overall weight. In a double-blind experiment, a leading researcher gave 21 patients with type II diabetes a daily dose of 8 grams of either a CLA mixture or placebo. After two months, the researchers correlated the subjects’ CLA blood levels with changes in their weight. They discovered that the t10c12 isomer—but not the c9t11 isomer—was significantly associated with weight loss.10 Intriguingly, the t10c12 isomer, which scientists believe may exert the most potent effects on body composition, is also associated with a decrease in leptin, a hormone associated with the body’s hunger-satiety feedback mechanism. Interestingly, the amount of CLA used in this study, 8 grams per day, is more than twice the amount given to subjects in trials in which no significant fat loss was reported.10
Other research, however, indicates that 2-4 grams of CLA taken daily may be enough to trigger fat loss. Several Scandinavian studies over the last five years offer evidence that relatively low doses of CLA reduce body fat, if not overall weight. A Norwegian study documented significant body-fat loss with just 1.8 grams of CLA per day. This double-blind, placebo-controlled, randomized study of 20 healthy, normal-weight people lasted three months. Subjects performed standardized exercises for 90 minutes, three times a week. They consumed either a placebo or 1.8 grams of a commercially available CLA mixture, which featured the c9t11 and t10c12 isomers in equal amounts. Subjects taking CLA experienced significant reductions in body fat, as measured by near-infrared light, and the CLA was well tolerated. The placebo subjects, by contrast, showed no change in body fat.18
Another Norwegian controlled study enrolled 60 overweight or obese subjects who were randomly assigned to one of five groups and received daily doses of 1.7-6.8 grams of CLA or placebo. Body fat was measured by dual-energy X-ray absorptiometry at baseline and then at six and 12 weeks. Scientists found a significantly greater body-fat loss among the groups taking 3.4 grams or more of CLA compared to the control subjects. Intriguingly, the group receiving 1.7 grams of CLA experienced no significant body-fat loss, nor did the groups taking more than 3.4 grams of CLA see any additional body-fat loss.19 Researchers concluded that a dose of 3.4 grams results in significant body-fat loss compared to placebo, but that greater amounts of CLA do not result in greater fat loss. The CLA was well tolerated and adverse events were no different among the placebo and various CLA groups. Forty-seven subjects completed the three-month study. Blood lipids and other blood-profile health parameters were unchanged among all groups.19
Slimming News from Sweden
A study in Sweden enrolled 53 healthy men and women who were randomly assigned to receive either 4.2 grams of CLA a day or an equal amount of placebo. Neither the subjects nor the researchers in this double-blind trial knew who was taking which substance. After three months, CLA subjects experienced a significant decrease in their proportion of body fat compared to control subjects. Weight, body mass index, blood glucose, and lipids remained unchanged for both groups. The researchers concluded that CLA reduces body fat without affecting other health parameters.20
A recent report from the University of Wisconsin confirmed CLA’s safety. Obese people who were otherwise healthy received either 6 grams daily of CLA or a placebo for one year. General blood chemistries revealed no adverse effects on liver function, blood glucose, lipids, or insulin levels from long-term CLA supplementation.21
Although the research results remain somewhat mixed, CLA supplementation appears to be safe and potentially beneficial for people seeking to trim body fat.
Battling Cancer at Every Turn
Ironically, the potent cancer-fighter CLA was first identified in fried and grilled beef, foods that are better known for containing cancer-causing substances (such as heterocyclic amines) than for supplying beneficial anti-carcinogens.1,22,23 Yet that is precisely where scientists at the University of Wisconsin first discovered the conjugated isomers they dubbed CLA.1
The Wisconsin scientists discovered that one or more of the isomers inhibit mutations in bacteria. Researchers believe that these mutations, which are generally damaging alterations to the genetic code, underlie the development of many cancers. They wondered whether CLA could offer protection against an experimentally induced laboratory model of skin cancer, and soon turned their attention to a rodent model of human cancer.1
The researchers enlisted the aid of rodents specially bred for their susceptibility to developing skin cancer when provoked with an appropriate toxin. One group of the cancer-prone mice was pre-treated with a synthetic mixture of four CLA isomers. Control groups of mice were pre-treated with either linoleic acid or an organic solvent. The researchers then applied a known carcinogenic chemical to the skin of all the test animals. It was soon apparent that CLA provides considerable protection against experimentally induced tumors, as the control subjects developed twice as many tumors as the CLA-treated mice.1