Life Extension Magazine September 2005
Why Lutein and Zeaxanthin Are Becoming So Popular
By Heather S. Oliff, PhD
For decades, scientists have tried to determine why people who eat more vegetables and fruits have such low rates of degenerative disease.
After evaluating hundreds of carotenoids found in red, yellow, and orange plants, researchers have zeroed in on lutein and zeaxanthin. They have discovered that these two carotenoids provide broad-spectrum effects, including protection against atherosclerosis and a reduced risk of certain cancers.
The benefit that doctors are focusing on most is the ability of these carotenoids to help maintain the structure of the macula. Considering the magnitude of today’s macular degeneration epidemic, it is no wonder that so many aging people are taking steps to ensure that they obtain enough lutein and zeaxanthin.
Lutein and Zeaxanthin Defined
Lutein and zeaxanthin are classified as xanthophyll carotenoids. Zeaxanthin is the stereoisomer of lutein—that is, it possesses the same chemical structure but in a different configuration. As such, lutein and zeaxanthin are often measured and reported as a single group, though they may have different functions in the body. Unlike other carotenoids, lutein and zeaxanthin are not sources of vitamin A because they are not converted to vitamin A; rather, lutein and zeaxanthin are lipid-soluble antioxidants. Lutein and zeaxanthin are found in the liver, ovaries, pancreas, kidneys, spleen, testes, adrenals, and many eye tissues. Some of these sites use the xanthophylls, while others may store them for future use.1
Sources of Lutein and Zeaxanthin
The most important sources of lutein and zeaxanthin are fruits and vegetables. While most people associate lutein and zeaxanthin with dark green, leafy vegetables, they are found in high concentrations in a rainbow of fruits and vegetables, and in egg yolks, as shown in Table 1.
Of 35 fruits and vegetables examined for their lutein and zeaxanthin content, corn was found to have the greatest quantity of lutein and orange pepper the most zeaxanthin.2 Lutein is found in many fruits and vegetables, while zeaxanthin is present in only a small number of fruits and vegetables.2 In fact, most dark green vegetables contain only traces of zeaxanthin.2 Cooking green, leafy vegetables reduces their lutein content,3 while eating whole spinach leaves rather than chopped spinach improves lutein absorption from the intestinal tract.4 One study, however, found that vegetable juice provides better lutein absorption than raw or cooked vegetables.5 The greatest quantities of lutein and zeaxanthin are found in egg yolk.2
Because most Americans do not eat enough fruits and vegetables—and a considerable number are leery of eating too many egg yolks—many people may not be obtaining enough xanthophylls through dietary sources. A recent study reported that cheddar cheese can be successfully fortified with lutein.6 Just as milk fortified with vitamin D increased vitamin D consumption, cheese fortified with lutein may boost lutein consumption. Lutein and zeaxanthin can also be consumed as dietary supplements.
The availability of carotenoids in the plasma is vital for long-term maintenance of adequate tissue levels of carotenoids.2 Plasma levels of micronutrients are correlated to the risk of certain disorders.2 For example, low plasma levels of lutein and zeaxanthin are associated with an increased risk of age-related macular degeneration (ARMD).7 Serum levels of lutein and zeaxanthin may be altered by diet, lifestyle, and physiology.8 The Third National Health and Nutrition Examination Survey (1988-1994) examined 7,059 Americans who were at least 40 years old.8 The survey determined that lower serum lutein and zeaxanthin levels are found in women, Caucasians, smokers, heavy drinkers, the physically inactive and overweight, those with a high waist-to-hip ratio, and those who have a lower dietary intake of lutein and zeaxanthin.8 Fortunately, nearly all of these factors can be modified by making healthy lifestyle changes that enhance serum levels of lutein and zeaxanthin.
Understanding the mechanism of action of lutein and zeaxanthin helps to explain their value for cancer prevention, cardiovascular health, and healthy eyes. Lutein and zeaxanthin are antioxidants that destroy harmful free radicals generated by exposure to light,9 which initiates oxidative damage in the eyes and skin. These xanthophylls provide protection against free radicals that can damage cells and DNA, and thus cause cancer.
Lutein and zeaxanthin may also improve the cytotoxic action of anti-cancer chemotherapy drugs.10 Multidrug resistance proteins, which are found in most malignant tumors, are important contributors to chemotherapy drug failure. Researchers studying lymphoma and breast cancer cells discovered that lutein inhibits the action of multidrug resistance proteins,10 thus enhancing chemotherapy. They also found that zeaxanthin could induce apoptosis (death) of the cancer cells.10 While more research is needed, this exciting new finding provides hope for better therapeutic outcomes.
Malignant melanoma is the most dangerous type of skin cancer, and its incidence is rapidly increasing. Risk factors include sun exposure and having a fair complexion that burns easily.
One of the largest case-controlled studies investigating the association between diet and melanoma reported that high intake of lutein from fruits and vegetables significantly reduces the risk of melanoma.11 Scientists believe these xanthophyll carotenoids prevent melanoma by protecting the skin against sun damage.11 Because tissue levels rather than plasma levels of xanthophylls provide this protection, eating lutein-containing foods on the day of sun exposure may not necessarily be protective, but daily consumption of such foods will be beneficial.
Because lutein and zeaxanthin are deposited in breast tissue, scientists are studying possible associations between breast cancer prevention and xanthophylls. While the study findings to date are inconclusive, some researchers have reported that lutein and zeaxanthin can protect against breast cancer.
A long-term study of 83,234 healthy women evaluated the relationship between breast cancer and carotenoid intakes.12 The researchers found that intake of lutein and zeaxanthin from food and supplements may reduce the risk of breast cancer in premenopausal women. The association was particularly strong among women with a higher risk for breast cancer, as determined by a family history of the disease or alcohol use (defined as at least one alcoholic drink per day).12 The authors concluded that consuming fruits and vegetables high in lutein and zeaxanthin may reduce the risk of breast cancer among premenopausal women.12 Similarly, another study of 540 women found an increased risk of breast cancer in women with very low intakes of lutein due to a poor diet or lack of supplementation.13
A major contributor to coronary heart disease is atherosclerosis, which is initiated by free radical oxidation of low-density lipoprotein (LDL) and causes arteries to occlude (close up).14 This oxidation damages the endothelial cells lining the arterial walls. The damaged cells subsequently initiate an inflammatory response and the release of adhesion molecules, which attract cells that form fatty plaques in the artery.14
A study using human aortic endothelial cells found that lutein can inhibit the expression (molecular formation) of adhesion molecules on the cell surface.15 Therefore, both this mechanism and lutein’s antioxidant capability could enable lutein present in plasma to protect against atherosclerosis. In fact, research on humans and mice has found that increased lutein intake protects against the progression of early atherosclerosis.16 Plasma levels rather than tissue levels of lutein are important for cardiovascular health, and thus regular dietary intake of lutein may protect against the progression of atherosclerosis.
Healthful Egg Yolks
Chicken egg yolks are the most abundant dietary source of lutein and zeaxanthin, but many people remain concerned about the cholesterol content of egg yolks. LDL cholesterol is a known contributor to atherosclerosis. Cholesterol, triacylglycerol, and phospholipids make up the yolk’s lipid matrix, and lutein and zeaxanthin are dispersed within this matrix.17 The yolk’s lipid matrix permits the body to absorb lutein and zeaxanthin efficiently.17 While consuming either 1.3 cooked egg yolks or 60 grams of cooked spinach daily increases plasma lutein levels to a similar amount, egg yolk intake also increases plasma zeaxanthin levels while spinach intake does not.17 Interestingly, one study reported that corn, which is very high in zeaxanthin, does not increase plasma zeaxanthin.17 The bioavailability of lutein in egg yolk may be greater than that of lutein and lutein ester supplements.18
Many recent studies report that daily egg consumption does not increase risk for coronary heart disease.19 Further supporting this finding is that while egg intake has declined over the past 30 years, coronary heart disease is still the leading cause of death in the US.19 Hence, the nutritional benefits of consuming egg yolk may simply outweigh related concerns about dietary cholesterol intake.19 For healthy individuals, consuming egg yolks may be beneficial by increasing lutein and zeaxanthin intake,2 and may help prevent atherosclerosis. While egg yolk is no longer considered a “forbidden food,”19 consuming 1.3 egg yolks per day is not recommended for people with elevated cholesterol or established coronary heart disease, as this could increase plasma cholesterol and risk for ischemic heart mortality.
Most people associate carrots (a rich source of beta-carotene) with eye health. Carrots are important for vision, though beta-carotene has not been detected in the eye lens, which is important for focusing vision.20 In fact, lutein and zeaxanthin are the predominant carotenoids in the eye lens and macula, the area in the eye’s retina that contains cells that are important for color vision.21 For this reason, lutein and zeaxanthin play a critical role in maintaining normal vision.
In the eye, lutein and zeaxanthin are known as the macular pigments, which are important for absorbing visible light, reducing light scatter in the eye, and providing antioxidant protection. Light is necessary for vision, but is also dangerous—capable of destroying cells or their components by photo-oxidation.22 Lutein and zeaxanthin can filter the high-energy blue and blue-green wavelengths of light from the visible-light spectrum.9 Filtering blue light has two important functions: it reduces chromatic aberration (poor image color) and prevents blue light-induced oxidation. In other words, these blue wavelengths, which allow a person to see the color blue, are similar to the invisible ultraviolet A and B wavelengths in that they can induce cell damage in the eyes. Phototoxic damage contributes to the pathophysiology of macular degeneration and cataract.