|LE Magazine October 1999 |
Continued from :
I3C Indole 3 carbinol The Tamoxifen Substitute Cancer Prevention For Thinking People.
And more. . .
In 1997, researchers at Strang Cancer Research Laboratory at Rockefeller University discovered that when I3C changes "strong" estrogen to "weak," it stops human cancer cells from growing (54-61%) and provokes the cells to self-destruct (apoptosis). Subsequent studies done at the University of California at Berkeley, show that I3C inhibits MCF7 human breast cancer cells from growing by as much as 90% in culture. Growth arrest does not depend on estrogen receptors.
I3C does more than just turn strong estrogen to weak. 16alpha-hydroxyestrone (16OHE) is an estrogen metabolite that is biologically active-i.e., like estradiol, it can send "grow" signals. In breast cancer, the bad 16OHE is elevated, and the good 2OHE is decreased. Cancer-causing chemicals change the metabolism of estrogen so that 16OHE is elevated. I3C changes them back so that 2OHE is increased.
In an experiment at New York University, researchers gave African-American women I3C, 400 mg for five days. Most of them experienced an increase in the "good" 2OHE and a decrease of the "bad" 16OHE. However, some did not. It turns out that those who did not have a mutation in a gene that helps metabolize estrogen to the 2OHE version. Those women have an eight times higher risk of breast cancer.
The I3C receptor
A startling discovery shows that I3C controls estrogen metabolism through the same receptor that allows dioxin into the cell-the "Ah" receptor (aryl hydrocarbon). Ah is similar to the estrogen receptor in that it can induce cellular growth. Unlike the estrogen receptor, however, scientists haven't found the body's natural "Ah" that fits into the Ah receptor. The only substances known to activate Ah are certain phytochemicals, including I3C-and the proven cancer promoter, dioxin. Dioxin is a chemical made from chlorine. It's so toxic that scientists measure it in trillionths of a gram. It's used in all kinds of things-from Saran Wrap(r) to pesticides to wood preservative. It has been detected in McDonald's Big Macs(r), Haagen-Daz(r) ice cream and Kentucky Fried Chicken(r). Meat, dairy products and fish are the most concentrated sources. When paper is bleached or plastic is burned, dioxin is released into the environment. Because it lodges in fat, it's almost impossible to remove from the human body. Losing weight simply causes it to hunker down in the remaining fat.
A study on people who worked in a dioxin plant shows that women exposed to the chemical have more than twice the risk of breast cancer, but some studies don't show any association at all. Part of the problem with dioxin studies is that there are hundreds of similar chemicals, with hundreds of different metabolites that may interact in ways we don't currently understand.
Dioxin, like I3C, affects estrogen metabolism. For this reason, it has been called an estrogen blocker (like tamoxifen). But it esn't work through the estrogen receptor. Dioxin and I3C both affect estrogen metabolism through the Ah receptor. But just as tamoxifen sends a different signal that genuine estrogen when it gets in the estrogen receptor, so does dioxin send a different signal than I3C in the Ah receptor. In addition to changing the metabolism of estrogen, dioxin also disrupts other important growth regulatory factors. Among those factors are insulin, IGF-1 (insulin-like growth factor), and tumor necrosis factor (TNF). It also activates cancer genes and suppresses tumor suppressor genes.
I3C, on the other hand, fits into the Ah receptor, but instead of sending signals that help cancer grow, it sends signals that stop it. I3C uses the Ah receptor to indirectly affect estrogen metabolism also, but in a beneficial way. Not only does it positively affect estrogen, it can also keep dioxin out of cells. When researchers at Texas A & M University treated breast cancer cells with I3C and dioxin at the same time, dioxin's adverse effects were reduced 90% by I3C.
I3C prevents chemically-induced breast cancer in rodents by 70-96%. It also prevents other types of cancer, including aflatoxin-induced liver cancer, leukemia and colon cancer. Studies show that I3C inhibits free radicals, particularly those that cause the oxidation of fat.
I3C stops cancer cells from growing
I3C not only weakens estrogen and keeps chemicals out of cells, it also goes after cancer in ways similar to tamoxifen. It, like tamoxifen, interrupts the cell cycle. In studies from the University of California mentioned above, I3C inhibited the growth of estrogen receptor-positive breast cancer cells by 90% compared to tamoxifen's 60% by stopping the cell cycle. (Adding tamoxifen to I3C gave a 5% boost.) In estrogen receptor-negative cells I3C stopped the synthesis of DNA for new cells by about 50% whereas tamoxifen had no significant effect. I3C also restores p21 and other tumor suppressors that act as check points during synthesis of a new cell. Tamoxifen, by contrast, has no effect on p21. I3C also inhibits cancers caused by other chemicals, in addition to dioxin. If animals are fed I3C before exposure to certain other cancer-causing chemicals, DNA damage and cancer will be virtually eliminated. A study on rodents shows that damaged DNA in breast cells is reduced 91% by I3C. Similar results happen in the liver. And in a study from New York University Medical Center, female smokers taking 400 mg of I3C significantly reduced their levels of a major lung carcinogen. Chemicals in cigarrettes are known to affect estrogen metabolism.
While there is no proven breast cancer preventive, the best and most comprehensive scientific evidence so far stands behind phytochemicals such as I3C. I3C beat out more than 80 other substances, including tamoxifen, for anti-cancer potential in an assay done at the National Cancer Institute.
Recently, researchers at the Hoechst Marrion Roussel drug company staked patent claims to dozens of indole-3 look-alikes. They claim that the indoles, which down-regulate estrogen receptors, can be used to treat and prevent cancer and autoimmune diseases such as multiple sclerosis, arthritis and lupus. They hope to replace all the chemically-altered estrogen drugs such as tamoxifen with a new generation of chemically-altered indole drugs that fit in the Ah receptor, and regulate estrogen indirectly. Will the fake indoles create cancer in other organs as tamoxifen does? Will they lead to chemical tumor dependency as tamoxifen does?
Time will tell if the constant stream of chemical lookalikes will continue to stop/feed cancer. In the meantime, those wishing to get off the chemical merry-go-round, and get serious cancer prevention without the side effects have a terrific option: I3C.Inhibition of growth in estrogen
receptor-positive breast cancer cells
we cannot say that I3C will absolutely prevent breast cancer. The studies that would allow us to give you the absolute proof haven't been done.
Currently there are two government-sponsored trials underway involving estrogen metabolism and vegetables. One is going to assess the "interactive effects of dietary fat and fruits and vegetables on the levels of oxidative DNA damage and cholesterol oxides in women at high risk for breast cancer." This study is enrolling a grand total of 160 women in the next two and a half years. The other plans to look at how fiber affects estrogen metabolism in postmenopausal women. This study is enrolling a phenomenal 40 women over four years. Should the government ever get serious about preventing breast cancer, and spend $100 million of your tax dollars on testing I3C as they have on testing tamoxifen, we would be able to give you the proof. For now, we can only give you the best evidence and let you decide.
Note that while a little is good, a lot is not necessarily better. As with certain antioxidants that can actually promote oxidation at high levels, too much I3C can have the opposite effect of what you want. Therefore, don't exceed the dosage. The effective dose established in human studies is 6-7 mg per kg of weight per day. For a 120 lb. woman, this is just under 400 mg/day.
Also note that pregnant women should not take I3C, due to its modulation of estrogen. The reported aversion to cruciferous vegetables by pregnant women may be associated with their ability to change estrogen metabolism.
INDOLE 3 CARBINOL
Anonymous. 1997. Tamoxifen has a characteristic mutation spectrum in human endometrial cancers (Meeting abstract). Proc Annu Meet Am Assoc Cancer Res 38:A3008.
Arnao MB, et al. 1996. Indole-3-carbinol as a scavenger of free radicals. Biochem Mol Biol Int 39:1125-34. Barakat RR, et al. 1997. Evaluation of p53, Bcl-2 and c-Ki-ras status in endometrial cancers occurring after breast cancer with or without tamoxifen treatment (Meeting abstract). Proc Annu Meet Am Soc Clin Oncol 16:A1289.
Bitonti AJ, et al. 1999. US Patent 5,877,202. Bradlow HL, et al. 1991. Effects of dietary indole-3-carbinol on estradiol metabolism and spontaneous mammary tumors in mice. Carcinogenesis 12:1571-74.
Chen I, et al. 1996. Indole-3-carbinol and diindolylmethane as aryl hydrocarbon (Ah) receptor agonists and antagonists in T47D human breast cancer cells. Biochem Pharmacol 51:1069-76.
Chen I, et al. 1998. Aryl hydrocarbon receptor-mediated antiestrogenic and antitumorigenic activity of diindolylmethane. Carcinogenesis 19:1631-9.
Cover CM, et al. 1998. Indole-3-carbinol inhibits the expression of cyclin-dependent kinase-6 and induces a G1 cell cycle arrest of human breast cancer cells independent of estrogen receptor signaling. J Biol Chem 273:3838-47.
Cover M, et al. 1999. Indole-3-carbinol and tamoxifen cooperate to arrest the cell cycle of MCF-7 human breast cancer cells. Cancer Res 59:1244-51.
Custodio JB, et al. 1994. Tamoxifen and hydroxytamoxifen as intramembraneous inhibitors of lipid peroxidation. Evidence for peroxyl radical scavenging activity. Biochem Phearmacol 47:1989-98.
Devanaboyina U, et al. 1997. Effects of indole-3-carbinol (I3C) and phenethyl isothiocyanate (PEITC) on 7,12-dimethylbenz[a]anthracene (DMBA)-induced DNA adducts in rat mammary glands and liver (Meeting abstract). Proc Annu Meet am Assoc. Cancer Res 38:A2427.
Ferlini C, et al. 1999. Tamoxifen induces oxidative stress and apoptosis in oestrogen receptor-negative human cancer cell lines. Br J Cancer 79:257-63.
Grubbs CJ, et al. 1995. Chemoprevention of chemically-induced mammary carcinogenesis by indole-3-carbinol. Anticancer Res 15:709-16.Guillot C, et al. 1996. Alteration of p53 damage response by tamoxifen treatment. Clin Cancer Res 2:1439-44.
Gundimeda U, et al. 1996. Tamoxifen modulates protein kinase C via oxidative stress in estrogen receptor-negative breast cancer cells. Biol Chem 271:13504-14.
He YH, et al. 1997. Chemopreventive properties of indole-3-carbinol (I3C): inhibition of DNA adduct formation of the dietary carcinogen, 2-amino-1-methyl-6-pehnylimidazo [4,5-b]pyridine (PhIP), in female F344 rats. J Cell Biochem Suppl 27:42-51.
Lim JS, et al. 1992. Tamoxifen suppresses tumor promoter-induced hydrogen peroxide formation by human neutrophils. Cancer Res 52:4969-72.
Manson MM, et al. 1998. Chemoprevention of afatoxin B1-induced carcinogenesis by indole-3-carbinol in rat liver-predicting the outcome using early biomarkers. Carcinogenesis 19:1829-36.
Manz, et al. Cancer mortality among workers in chemical plant contaminated with dioxin. Lancet 338:959-64.
Michnovicz JJ, et al. 1991. Altered estrogen metabolism and excretion in humans following consumption of I3C. Nutr Cancer 16:59-66.
Michnovicz JJ, et al. 1997. Changes in levels of urinary estrogen metabolites after oral I3C treatment in humans. J Natl Cancer Inst 89:718-23.
Michnovicz JJ. 1998. Increased estrogen 2- hydroxylation in obese women using oral indole-3-carbinol. Int J Obes Relat Metab Disord 22:227-9.
Norris JD, et al. 1999. Peptide antagonists of the human estrogen receptor. Science 285:744-46. Paige LA, et al. 1999. Estrogen receptor (ER) modulators each induce distinct conformational changes in ER alpha and ER beta. Proc Natl Acad Sci USA 96:3999-4004.
Ricci MS, et al. 1999. Estrogen receptor reduces CYP1A1 induction in cultured human endometrial cells. J Biol Chem 274:3430-38.
Schecter A, et al. 1997. Dioxins, dibenzofurans, dioxin-like PCBs and DDE in U.S. fast food, 1995. Chemosphere 34:1449-57.
Sharma S, et al. 1994. Screening of potential chemopreventive agents using biochemical markers of carcinogenesis. Cancer s 54:5848-55.
Shertzer HG, et al. 1988. Intervention in free radical mediated hepatotoxicity and lipid peroxidation by indole-3-carbinol. Biochem Pharmacol 37:333-38.
Taioli E, et al. 1997. Effects of indole-3-carbinol (I3C) on the metabolism of the tobacco-specific lung carcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) in smokers (Meeting abstract). Proc Annu Meet am Assoc Cancer Res 38:A1413.
Taioli E, et al. 1999. Role of estradiol metabolism and CYP1A1 polymorphisms in breast cancer risk. Cancer Detect Prev 23:232-7.
Telang NT, et al. 1997. Inhibition of proliferation and modulation of estradiol metabolism: novel mechanisms for breast cancer prevention by the phytochemical I3C. Proc Soc Exp Biol Med 216:246-52.
Thangaraju M, et al. 1994. Effect of tamoxifen on lipid peroxide and antioxidative system in postmenopausal women with breast cancer. Cancer 74:78-82.
Vancutsem PM, et al. 1994. Frequent and specific mutations of the rat p53 gene in hepatocarcinomas induced by tamoxifen. Cancer Res 54:3864-7.
Verhagen H, et al. 1995. Reduction of oxidative DNA-damage in humans by brussels sprouts. Carcinogenesis 16:969-70.
Verhoeven DT, et al. 1996. Epidemiological studies on brassica vegetables and cancer risk. Cancer Epidemiol Biomarkers Prev 733-48.
Wattenberg LW, et al. 1978. Inhibition of polycyclic aromatic hydrocarbon-induced neoplasia by naturally occurring indoles. Cancer Res 38:1410-13.
Wei H, et al. 1998. Tamoxifen reduces endogenous and UV light-induced oxidative damage to DNA, lipid and protein in vitro and in vivo. Carcinogenesis 19:1013-8.
Yang JH, et al. 1999. A malignant transformation of human cells by 2,3,7,8-tetrachlorodibenzo-p-dioxin exhibits altered expression of growth regulatory factors. Carcinogenesis 20:13-18.